Below are shown three Lineweaver-Burk plots for enzyme reactions that have been carried out in the presence, or absence, of an inhibitor.

You are watching: Indicate what type of inhibition is predicted based on lineweaver-burk plot.

Part A

Indicate what type of inhibition is predicted based on Lineweaver-Burk plot.

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 mixed inhibition
 competitive inhibition
 uncompetitive inhibition

 

Part B

Indicate which line corresponds to the reaction without inhibitor and which line corresponds to the reaction with inhibitor present.

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 red - without inhibitor, blue - with inhibitor
 blue - without inhibitor, red - with inhibitor

 

Part C

Indicate what type of inhibition is predicted based on Lineweaver-Burk plot.

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 mixed inhibition
 competitive inhibition
 uncompetitive inhibition

 

Part D

Indicate which line corresponds to the reaction without inhibitor and which line corresponds to the reaction with inhibitor present.

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Indicate which line corresponds to the reaction without inhibitor and which line corresponds to the reaction

 blue - without inhibitor, red - with inhibitor
 red - without inhibitor, blue - with inhibitor

 

Part E

Indicate what type of inhibition is predicted based on Lineweaver-Burk plot.

*

 mixed inhibition
 competitive inhibition
 uncompetitive inhibition

 

Part F

Indicate which line corresponds to the reaction without inhibitor and which line corresponds to the reaction with inhibitor present.

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 blue - without inhibitor, red - with inhibitor
 red - without inhibitor, blue - with inhibitor

Solutions


Expert Solution

Part-A

The lineweaver-Burk equation for the mixed inhibition is, (frac1v_0=left(fracalpha K_mV_max ight) frac1+fracalpha^primeV_max )

Here, (v_0) is the initial velocity, () is the substrate concentration,

$$ alpha^prime=left(1+fracK_I^prime ight), alpha=left(1+fracK_I ight) $$

() is the inhibitor concnetration, (V_max ) is the maximum velocity (K_I^prime) and (K_I^prime) are the dissociation constants, and (K_m) is the Michael"s constant.

The corresponding lineweaver-Burk plot for the mixed inhibition is shown below:

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Plot 1: Lineweaver-Burk plot in the presence of mixed inhibitor

The curled arrow which is in anti-clock wise direction indicates increasing inhibitor concentration.

Compare the specified plot with the plot-1. Thus, based on the plot the enzyme reaction is a mixed inhibition type.

Part-B

Compare the specified plot with plot-1, Thus, the blue line indicates the line equation without inhibitor and, the red line indicates the line equation with inhibitor.

Part-C

The lineweaver-Burk equation for the competitive inhibition is, (frac1v_0=left(fracalpha K_mV_max ight) frac1+frac1V_max )

The corresponding lineweaver-Burk plot for the competitive inhibition is shown below:

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Plot 2: Lineweaver-Burk plot in the presence of a competitive inhibitor

Compare the specified plot with the plot-2. Thus, based on the plot the enzyme reaction is competitive inhibition type.

Part-D

Compare the specified plot with plot-2, Thus, the blue line indicates the line equation without inhibitor and, the red line indicates the line equation with inhibitor.

Part-E

The lineweaver-Burk equation for the uncompetitive inhibition is, (frac1v_0=left(fracK_mV_max ight) frac1+fracalpha^primeV_max )

The corresponding lineweaver-Burk plot for the uncompetitive inhibition is shown below:

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Plot 3: Lineweaver-Burk plot in the presence of the uncompetitive inhibitor

Compare the specified plot with the plot-3. Thus, based on the plot the enzyme reaction is an uncompetitive inhibition type.

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Part-F

Compare the specified plot with plot-3, Thus, the blue line indicates the line equation without inhibitor and, the red line indicates the line equation with inhibitor.